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In the present study, three methods were used to assess the response of Tilapia zilli to filarial larvae. The first method involved the aspiration of microfilariae out of gills of fish previously exposed to infective larvae on the skin of a donor fish. The second method involved the aspiration of microfilariae directly from infected donor skin. The third method involved the aspiration of the lymphatic vessels drained from the fish’s skin with adult worms removed to provide a source of blood for the microfilariae. In all three methods, three-fourths of the fish exhibited a short-lived (aspiration method), post-parasitic diphasic contraction of the intestine which was best detected by maintaining the cavity of aspiration open for 10 min post-aspiration. It consisted of a first twitch followed by a second twitch. The first twitch was synchronous with the heart (electrocardiogram) of the fish. The first twitch occurred with a rise in temperature in all three methods. During the second twitch the temperature remained constant, in the aspiration method. In contrast, in the methods involving direct aspiration or aspiration from skin, the temperature of the fish remained constant during this phase. The first twitch remained even after the microfilariae were inactivated by bathing in paraformaldehyde solution. These results indicate that the first twitch is a reflex response occurring in the presence of a stimulus. The second twitch represents a somatic response to the stimulus.Hardee County High School

Hardee County High School (HC) is a public high school in LaFayette, Florida, United States. It opened in 1960, and is the only high school in the Hardee County School District. Its mascot is the Patriot.

History
The school was first located in the city of LaFayette, but was built on a site adjacent to a local Country club. It is named for Gov. Joseph Hardee, who was instrumental in the settlement and founding of

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