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We determined the distribution of 5-hydroxytryptamine receptor-2C (5-HT2C) subtype on the basis of [3H]-GR125743 in the rat brain by quantitative autoradiography. [3H]-GR125743 bound to a single high-affinity class of sites with a KD of 2.5 nM and a Bmax of 32 fmol/mg protein in cortex, 41 fmol/mg protein in amygdala and 84 fmol/mg protein in hippocampus. Saturation experiments indicated that the sites were 5-HT2C subtype. Specificity of [3H]-GR125743 binding was examined using various unlabelled compounds, and the autoradiographic distribution of [3H]-GR125743 binding was determined in rats pretreated with (+/-)-1-phenyl-2,3,4,5-tetrahydro-1H-3-benzazepin-7 hydrochloride (LY341495) or cyproheptadine. In addition, the effect of GR125743 on [3H]-GR125743 binding was examined in sections from rats treated with (+/-)-1-phenyl-2,3,4,5-tetrahydro-1H-3-benzazepin-7 hydrochloride. In sections from the cortex, binding was localized to the dentate gyrus, hippocampus, thalamus and globus pallidus. In sections from the amygdala, [3H]-GR125743 binding was localized to the bed nucleus of the stria terminalis, the lateral, medial and accessory basal amygdaloid nuclei, the central nucleus of the amygdala and the medial amygdaloid nucleus. In sections from the hypothalamus, [3H]-GR125743 binding was localized to the magnocellular and parvocellular divisions of the paraventricular nucleus and the tuberomammillary nucleus.
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Windows 7 Loader 2.0.9 and Windows 7 Activator Reloaded Final v2.0.9/*
* Copyright (c) 2014-2016 Erik Doernenburg and contributors
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@interface OCMPostSetPropertyStub : NSObject